Molecular Genetics

Completion:examination (written&verbal)
Type of
Hour/semLecturesSem. ExercisesLab. exercisesComp. exercisesOther
Guarantee:Doškař Jiří, prof. RNDr., CSc., FCH
Lecturer:Doškař Jiří, prof. RNDr., CSc., FCH
Faculty:Faculty of Chemistry BUT
Learning objectives:
  To understand the basic knowledge of genetic information, genes and genomes, expression of genes and its regulation. To understand the methods used in molecular genetics and gene engineering in research, industry, agriculture and medicine.
  The course is an introduction into molecular biology and genetics. It describes the organization of genetic information  of procaryotic and eucaryotic genomes. It explains the principles of transcription and translation of genetic information and regulation of gene expression. Molecular mechanisms of mutagenesis and recombination, repair mechanisms of damaged DNA and mobile elements are also presented. The second part of the lecture is focused on the basic methods of molecular genetics and their applications in gene engineering in industry, agriculture and health care delivery.
Knowledge and skills required for the course:
  The knowledge of biology and chemistry at the level of a high school study programme.
Subject specific learning outcomes and competences:
  Students will obtain the basic knowledge of genetic information,  expression of genes, its regulation and the methods allowing to modify genetic information.
Generic learning outcomes and competences:
  Understanding the field which is not the main study component in a computer science-based study programme.
Syllabus of lectures:
  1. History of molecular genetics
  2. Genetic information and the genetic code
  3. Molecular structure of procaryotic and eucaryotic genome
  4. Replication and transcription of procaryotic and eucaryotic genome
  5. Postranscriptional modification and processing of RNA
  6. Translation and posttranslational processing
  7. Regulation of gene expression
  8. Molecular mechanisms of mutagenesis and recombination. Repair mechanisms of damaged DNA
  9. Mobile elements 
  10. Basic methods of molecular genetics 
  11. Basics of gene engineering
  12. Gene therapy. Genetic diseases and their diagnostics.
  13. Applications of molecular genetic
Fundamental literature:
  • Šmarda J. a kol.. Metody molekulární biologie, Brno, 2005 (in Czech).
  • Lewin B. Genes VII, Oxford University Press, Oxford, New York, Tokyo 2002.
  • Alberts et al.: Molecular biology of the cell. Garland Publ. 2004.
  • Clark D.: Molecular biology, Elsevier, 2005.
  • Watson J.D. et al., Recombinant DNA, 2nd ed., W.H. Freeman, New York 1992.
Study literature:
  • Rosypal S. a kol.: Úvod do molekulární biologie. I.-IV. díl. Brno 1999-2002 (3rd ed.), 2006 - I. díl (4th ed.), in Czech.
  • Rosypal S. a kol.: Terminologie molekulární biologie, Brno 2001 (in Czech).
  • Alberts a kol: Základy buněčné biologie, Espero, 2000, 2005 (in Czech).